SOPs for Drug Discovery

The purpose of this Standard Operating Procedure (SOP) is to outline the process of purifying proteins for structural studies in drug discovery. Protein purification is a critical step in the study of biomolecular structures and interactions, enabling the analysis of protein-ligand binding, enzymatic activity, and other molecular properties. This SOP ensures that protein purification is performed systematically and consistently to obtain high-quality protein samples suitable for downstream structural studies, such as X-ray crystallography, NMR spectroscopy, or cryo-EM.
Click to read the full article.

The purpose of this Standard Operating Procedure (SOP) is to describe the use of Differential Scanning Calorimetry (DSC) for studying the thermal properties of drug candidates and their interactions with biological macromolecules. DSC is a technique that measures the heat required to increase the temperature of a sample and provides critical data about the stability, folding, and binding properties of molecules. This SOP ensures that DSC experiments are performed consistently, and the resulting data are interpreted correctly for use in drug discovery, particularly in the context of understanding ligand-target interactions and compound stability.
Click to read the full article.

The purpose of this Standard Operating Procedure (SOP) is to describe the process of using Isothermal Titration Calorimetry (ITC) for studying molecular interactions in drug discovery. ITC is a powerful technique used to directly measure the thermodynamic parameters of biomolecular interactions, including binding affinity, enthalpy, entropy, and stoichiometry. This SOP ensures that ITC studies are performed consistently, with accurate data generated to support lead compound identification and optimization in drug discovery.
Click to read the full article.

The purpose of this Standard Operating Procedure (SOP) is to outline the use of Surface Plasmon Resonance (SPR) for studying molecular interactions in drug discovery. SPR is a highly sensitive technique used to measure real-time binding kinetics, affinity, and specificity of interactions between a ligand (e.g., small molecule, peptide) and a target (e.g., protein, receptor). This SOP ensures that SPR studies are performed consistently and accurately to obtain valuable data for the identification and optimization of lead compounds in drug discovery.
Click to read the full article.

The purpose of this Standard Operating Procedure (SOP) is to describe the use of biophysical methods in drug discovery. Biophysical techniques are essential tools for understanding the interaction between drug candidates and biological targets, providing crucial information about binding kinetics, affinity, and molecular conformation. This SOP ensures that biophysical methods are applied systematically, reliably, and consistently to accelerate drug discovery by providing high-quality data for the optimization of lead compounds.
Click to read the full article.

The purpose of this Standard Operating Procedure (SOP) is to describe the process for validating assay methods used in drug discovery. Assay validation is a critical step to ensure the reliability, reproducibility, and robustness of assays employed in screening potential drug candidates. This SOP ensures that assays meet required performance standards, providing high-quality data for further optimization and decision-making in drug discovery.
Click to read the full article.

The purpose of this Standard Operating Procedure (SOP) is to describe the process for developing assays for target screening in drug discovery. Assay development is crucial for evaluating the interaction between drug candidates and biological targets, such as enzymes, receptors, or other molecular entities. This SOP ensures that assays are developed efficiently, with high sensitivity and reproducibility, to facilitate the identification of potential drug candidates for further optimization and development.
Click to read the full article.

The purpose of this Standard Operating Procedure (SOP) is to outline the process for isolating natural products from plant, microbial, or marine sources for use in drug discovery. Natural products have long been a source of novel compounds with therapeutic potential. This SOP ensures that the isolation process is conducted systematically, efficiently, and safely to identify bioactive natural products that may serve as lead candidates for drug development.
Click to read the full article.

The purpose of this Standard Operating Procedure (SOP) is to describe the process of microbial screening for antimicrobial compounds in drug discovery. Microbial screening involves evaluating the antimicrobial activity of compounds against a wide range of microorganisms to identify potential drug candidates for the treatment of infections. This SOP ensures that microbial screening is conducted systematically, with proper controls and data analysis, to identify promising antimicrobial agents for further development.
Click to read the full article.

The purpose of this Standard Operating Procedure (SOP) is to describe the proper handling, operation, and maintenance of high-throughput screening (HTS) equipment used in drug discovery. HTS is a technology that allows for the rapid testing of large numbers of compounds to identify potential drug candidates. This SOP ensures that HTS equipment is used safely and effectively to maximize the reliability of screening results, maintain equipment performance, and ensure compliance with safety and regulatory standards.
Click to read the full article.